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tp53 functional analysis  (Bio-Rad)
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Bio-Rad tp53 functional analysis
Sensitivity of NIH-OVCAR3 cells to VE-821, carboplatin and rucaparib. NIH-OVCAR3 cells were exposed to ( A ) VE-821 for 48 h, ( B ) carboplatin for 24 h or ( C ) rucaparib for 24 h. Media was replaced with drug free media for 21 days to allow colony formation. Data are the mean and standard deviation of at least three independent experiments. ( D ) Sensitivity of an additional 12 ovarian cancer cell lines to the three drugs was calculated in the same way and LC 50 values were calculated. Sensitivity of NIH-OVCAR3 cells in comparison to the mean of 12 other ovarian cancer cell lines, and the cell lines grouped by their HRR status into HRR competent (HRC, 3 cell lines) or HRR defective (HRD, 2 cell lines). Data are pooled from 3 independent experiments per cell line. Western blot confirming the loss of <t>TP53</t> function in NIH-OVCAR3 cells can be found at .
Tp53 Functional Analysis, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Sensitivity of NIH-OVCAR3 cells to VE-821, carboplatin and rucaparib. NIH-OVCAR3 cells were exposed to ( A ) VE-821 for 48 h, ( B ) carboplatin for 24 h or ( C ) rucaparib for 24 h. Media was replaced with drug free media for 21 days to allow colony formation. Data are the mean and standard deviation of at least three independent experiments. ( D ) Sensitivity of an additional 12 ovarian cancer cell lines to the three drugs was calculated in the same way and LC 50 values were calculated. Sensitivity of NIH-OVCAR3 cells in comparison to the mean of 12 other ovarian cancer cell lines, and the cell lines grouped by their HRR status into HRR competent (HRC, 3 cell lines) or HRR defective (HRD, 2 cell lines). Data are pooled from 3 independent experiments per cell line. Western blot confirming the loss of TP53 function in NIH-OVCAR3 cells can be found at .

Journal: Cancers

Article Title: Characterisation of Ovarian Cancer Cell Line NIH-OVCAR3 and Implications of Genomic, Transcriptomic, Proteomic and Functional DNA Damage Response Biomarkers for Therapeutic Targeting

doi: 10.3390/cancers12071939

Figure Lengend Snippet: Sensitivity of NIH-OVCAR3 cells to VE-821, carboplatin and rucaparib. NIH-OVCAR3 cells were exposed to ( A ) VE-821 for 48 h, ( B ) carboplatin for 24 h or ( C ) rucaparib for 24 h. Media was replaced with drug free media for 21 days to allow colony formation. Data are the mean and standard deviation of at least three independent experiments. ( D ) Sensitivity of an additional 12 ovarian cancer cell lines to the three drugs was calculated in the same way and LC 50 values were calculated. Sensitivity of NIH-OVCAR3 cells in comparison to the mean of 12 other ovarian cancer cell lines, and the cell lines grouped by their HRR status into HRR competent (HRC, 3 cell lines) or HRR defective (HRD, 2 cell lines). Data are pooled from 3 independent experiments per cell line. Western blot confirming the loss of TP53 function in NIH-OVCAR3 cells can be found at .

Article Snippet: Lysates were diluted to equal concentrations between 0.5–1 mg/mL in 2 × Laemmli buffer (BioRad, Hercules, CA, USA) for TP53 functional analysis, or XT sample buffer and XT reducing agent (BioRad) for VE-821 inhibition and baseline protein expression analysis, and boiled for 5 min at 95 °C.

Techniques: Standard Deviation, Comparison, Western Blot

Distribution of genomic and transcriptomic alterations of select DDR genes among 201 HGSOCs. Alteration frequencies of TP53 and DDR genes with amplification and deep deletion in NIH-OVCAR3 cell lines and additional DDR genes analysed as likely determinants of ATRi resistance in NIH-OVCAR3 cell line. Figure generated using cBioPortal ( https://www.cbioportal.org/ ).

Journal: Cancers

Article Title: Characterisation of Ovarian Cancer Cell Line NIH-OVCAR3 and Implications of Genomic, Transcriptomic, Proteomic and Functional DNA Damage Response Biomarkers for Therapeutic Targeting

doi: 10.3390/cancers12071939

Figure Lengend Snippet: Distribution of genomic and transcriptomic alterations of select DDR genes among 201 HGSOCs. Alteration frequencies of TP53 and DDR genes with amplification and deep deletion in NIH-OVCAR3 cell lines and additional DDR genes analysed as likely determinants of ATRi resistance in NIH-OVCAR3 cell line. Figure generated using cBioPortal ( https://www.cbioportal.org/ ).

Article Snippet: Lysates were diluted to equal concentrations between 0.5–1 mg/mL in 2 × Laemmli buffer (BioRad, Hercules, CA, USA) for TP53 functional analysis, or XT sample buffer and XT reducing agent (BioRad) for VE-821 inhibition and baseline protein expression analysis, and boiled for 5 min at 95 °C.

Techniques: Amplification, Generated